Technical repository

Technical Note #1
Date: December 5, 2025
Researcher: Zheng Cao, PhD

Stabilizing mutations for EBOV GP

GP1:
H39R
T60L
T60F
R64V
S65V
G67A
P146T
A152M
G173D
A222V

Technical Note #2
Date: December 5, 2025
Researcher: Rebekah Costello

Sudan GP N-Glycosylation Mutants for Hyperglycosylated Variants

1. Q264N, Q265K, L266T
2. N282T
3. A283N, D284L, I285S
4. D284N, I285G, G286T
5. D284N, I285G, G286S
6. E299N, Q300I, L301T
7. E299N, Q300V, L301T
8. L262N, Q264T
9. Q300N, L301S, R302S
10. Q300N, L301V, R302T
11. S307N, F308I, E309T
12. A310N
13. F308N, E309V, A310T
14. E309N, L311S
15. E309N, A310S, L311S

Technical Note #3
Date: December 15, 2025
Researcher: Katinka Alexandra Mitchell-Thomson

Engineering Ravn GP:

Stabilizing truncation:
Residues 436 to 483 

Yield-increasing mutants:
R597P, W598P, G599P, G600P, R575P, V576P, T577P, T578P, F583P, S584P, L585P, I586P, Q552P, N553P, N554P, L555P

Shielding glycan removal:
N171Q

Technical Note #4
Date: January 16, 2026
Researcher: Zheng Cao, PhD

Additional stabilizing mutations for EBOV glycoprotein (GP)

GP1:
D55V
N69L
W104V

Technical Note #5
Date: January 21, 2026
Researcher: Varun Shanker

Stabilizing mutations to SUDV GP:
K588I¹
I529A
Q552E
W615G
T577P
T576P
T583P

¹ Publicly disclosed on December 12, 2025.