Technical repository

Technical Note #1
Date: December 5, 2025
Researcher: Zheng Cao, PhD

Stabilizing mutations for EBOV GP

GP1:
H39R
T60L
T60F
R64V
S65V
G67A
P146T
A152M
G173D
A222V

Technical Note #2
Date: December 5, 2025
Researcher: Rebekah Costello

Sudan GP N-Glycosylation Mutants for Hyperglycosylated Variants

1. Q264N, Q265K, L266T
2. N282T
3. A283N, D284L, I285S
4. D284N, I285G, G286T
5. D284N, I285G, G286S
6. E299N, Q300I, L301T
7. E299N, Q300V, L301T
8. L262N, Q264T
9. Q300N, L301S, R302S
10. Q300N, L301V, R302T
11. S307N, F308I, E309T
12. A310N
13. F308N, E309V, A310T
14. E309N, L311S
15. E309N, A310S, L311S

Technical Note #3
Date: December 15, 2025
Researcher: Katinka Alexandra Mitchell-Thomson

Engineering Ravn GP:

Stabilizing truncation:
Residues 436 to 483 

Yield-increasing mutants:
R597P, W598P, G599P, G600P, R575P, V576P, T577P, T578P, F583P, S584P, L585P, I586P, Q552P, N553P, N554P, L555P

Shielding glycan removal:
N171Q

Technical Note #4
Date: January 16, 2026
Researcher: Zheng Cao, PhD

Additional stabilizing mutations for EBOV glycoprotein (GP)

GP1:
D55V
N69L
W104V

Technical Note #5
Date: January 21, 2026
Researcher: Varun Shanker

Stabilizing mutations to SUDV GP:
K588I¹
I529A
Q552E
W615G
T577P
T576P
T583P

¹ Publicly disclosed on December 12, 2025.

Technical Note #6
Date: March 31, 2026
Researcher: Katinka Alexandra Mitchell-Thomson

Stabilizing and antibody-affinity–enhancing mutations in Marburg:
V576P
N553P
S584P

Technical Note #7
Date: March 31, 2026
Researcher: Zheng Cao, PhD

Additional stabilizing mutations for EBOV glycoprotein (GP):
GP1:
R64I
R64L
T60I

EBOV GP N-Glycosylation Mutants for Hyperglycosylated Variants:
1. R219N Y220F Q221S
2. Q221N A222V T223T
3. T223N G224G F225S
4. Y220N Q221H A222T
5. E231N Y232F L233T
6. E245N S246D R247S
7. S246N R247E F248T
8. K265N R266S S267S
9. Y261N T262E S263S
10. S263N K265S
11. I274N W275L K276S
12. W275N K276E V277T
13. E280N I281V D282T
14. D282N T283E T284S
15. T283N T284E I285T
16. I285N E287S
17. R302N S303A E304S
18. E305N L306F S307S
19. K300N R302S
20. S303N E304D E305S